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Elements and Performance Criteria

1. Prepare for Anguina testing
   • Review test request to identify samples to be tested, required test methods, equipment and staff to be involved in the test
   • Clean and calibrate equipment and working space
   • Prepare the working sample or sub-samples using mixing and dividing processes
   • Record sample details on worksheets and sample packets
2. Inspect seed samples
   • Inspect a prescribed sample weight of ryegrass seed on a diaphanoscope for the presence of galls
   • Separate any galls present into yellow and black galls, count and report separately
3. Examine galls
   • Soak galls in water for prescribed period
   • Dissect galls and examine under a microscope for the presence of nematodes
   • Record the presence of nematodes
4. Report results
   • Review testing procedures carried out by analysts
   • Issue a report of ‘no galls found’ where no galls are found on the prescribed weight of seed
   • Report as ‘number of galls per sample weight’ where galls containing nematodes have been detected

Range Statement

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Performance Evidence

The candidate must be assessed on their ability to integrate and apply the performance requirements of this unit in a workplace setting. Performance must be demonstrated consistently over time and in a suitable range of contexts.

The candidate must provide evidence that they can:

identify galls on ryegrass seed

inspect seed samples using a diaphanoscope

plan and delegate testing tasks

use a microscope to detect nematode gall (Anguina spp.)

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Knowledge Evidence

The candidate must demonstrate knowledge of:

basic nematode identification

enterprise standard operating procedures (SOPs)

recording and reporting protocols

relevant health, safety and environment requirements

seed biology

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